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AUBF Lab
Hemocytometer
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Cards (27)
The specimen to be used in hemocytometer must be MIXED WELL for how long?
1-2 minutes
Specimens to be used for hemocytometer must be of what consistency?
Diluted
What should be used to clean a hemocytometer?
70% isoprophyl alcohol
in
gauze pad
When the hemocytometer is properly filled, the volume of the liquid in the chamber has a fixed depth of what?
0.1 mm
The number of squares counted on each chamber depends on what?
the total number of
cells
present
The accuracy in cell counts is DIRECTLY RELATED to what?
number of cells
The greater the number of cells, the greater the what?
accuracy
When not needed, what are avoided?
high dilutions
If there is EXTREMELY LOW CELL COUNTS, what can be done?
count additional squares
If there is already a HIGH CELL COUNT, what can be done?
count fewer cells
Dilutions are performed and analyzed in what?
duplicate cell counts
If there is an error in the preparation, we must what each chamber?
compare
Comparison must agree on what percentage?
less than
or
equal to 20%
If greater than 20%, what should be done?
repeat
DILUTION
and
counting
TRUE replication or not: counting the same chamber twice?
not true
TRUE replication or not: comparing chamber using same dilution?
not true
Which thoma has a SMALLER BULB??
Thoma WBC
What is the capacity of the bulb of THOMA WBC?
10mL
Which thoma has a BIGGER BORE?
THOMA WBC
What is the calibration of thoma WBC?
11
What is the beed color of thoma WBC?
WHITE
Which thoma has a BIGGER BULB?
thoma
RBC
What is the capacity of a thoma RBC?
100ml
which thoma has a smaller bore?
thoma rbc
what is the calibration of thoma RBC?
101
What color is the beed of thoma rbc?
red
Dilution are based on what?
visual appearance