common problems associated with routine blood specimen
absence of feather, holes in the smear, ridges or uneven thickness, smear too thick, smear too thin, smear too long, smear too short, streaks and trials in feathered edge
3 phases of laboratory examination
pre-examination, examination, post-examination
15 physiologic factors affecting the pre-examination phase
age, altitude, diet, dehydration, diurnal/circadian variations, drug therapy, exercise, environmental factors, fever, intramuscular injection, jaundice/icterus, pregnancy, smoking, stress, sex
allergies to supplies and equipment, apprehensive patients, excessive bleeding, fainting spells, nausea and vomiting, pain, petechiae, seizure/convulsion
procedural error risks
hematoma formation and bruising, iatrogenic anemia, infection, nerve injury, inadvertent arterial puncture, vein damage, reflex of additive
causes of nick puncture to the nerve
improper collection site, inserting the needle too deeply and quickly, excessive or lateral redirection of the needle, blind probing, patient startle reflex during needle insertion
procedural error and specimen quality concerns
hemoconcentration, hemolysis, partially-filled/over-filled tubes, specimen contamination, quantity not sufficient, wrong or expired collection tubes
preexamination phase includes
patient-related variables, sample collection and labelling techniques, patient preservatives/ anticoagulant, sample transport, processing and storage
10 common problems
misidentifiaction of patient, mislabeling of specimen, short draws/wrong anticoagulant/blood ration, wrong tubes/wrong anticoagulatn, mixing problems/clots, hemolysis/lipemia, hemoconcentration from prolonged tourniquet time, exposure to light/extreme temperatures, impoperly timed specimens/delayed delivery of specimens to the lab, processing errors: incomplete centrifugation, improper storage, incorrect log-in