Beetroot experiment

• independent various variable (beetroot experiment)


  Concentration of detergent
• Two abiotic varibles that should be controlled in this investigation (beetroot)

  Temperature

  pH
• Explain why the discs were removed carefully(beetroot)

  In order to avoid more damage to the docs which would cause more pigment release causing results to be invalid
• Explain why five discs were sued for each concentration of detergent (beetroot)

  To calculate SD and identify anomalies
• Dependent variable (beetroot)

  The percentage transmission of light(the absorption)
• Effect of temperature (beetroot)

  at first: increase in temperature results in more KE so the phospholipids in the membrane start to move around little bit more and becomes more fluid so more anthocyanin is able to escape and diffuse out
• Effects of temperature (beetroot)

  Mid way: above 40° there is a rapid increase the proteins of the membrane are starting to become denatured and membrane is starting to fall apart so all the anthocyanin is leaking out

  finally: it starts to plateau the membrane has completely disintegrated and anthocyanin has leaked out till it’s reached equilibrium