CRISPR-Cas9

• Description

  CRISPR-Cas 9: a technology used to cut DNA at a particular base so it can then be attached to a guide RNA that targets a specific complementary nucleotide sequence to which it will be added

  • Genes can be spliced and inserted with pinpoint accuracy
• Future directions: Multiplex Genome Editing


  Enables simultaneous editing of multiple genomic loci within the same cell.

  Benefit: facilitates the development of therapeutic strategies targeting multiple genes involved in disease pathways
• Future Directions: Epigenome editing


  enables targeted modification of epigenetic marks, such as histone modifications

  • Benefit: epigenome editing could provide insights into gene regulation mechanisms and offer new therapeutic avenues for diseases
• Future Directions: Base Editing


  techniques like base editing aim to precisely modify single nucleotides in the genome without inducing double-strand breaks

  Benefit: reduces off-target effects which can cause mutations and impact the organism
• Future Directions: Prime Editing


  Prime editing combines CRISPR-Cas9 technology with reverse transcriptase to directly rewrite genomic sequences

  Benefit: edit sequences of up to around 80 base pairs, making it suitable for larger-scale modifications