Gel Electrophoresis

• Gel electrophoresis is a powerful technique used to separate and visualize proteins or nucleic acids.
• Substances to be analyzed are driven along a gel of cross-linked molecular sieves using an electric current.
• The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is used to analyze proteins based on their molecular mass. It is therefore used to analyze the molecular weight of a given protein.
• Isoelectric focusing is a type of electrophoresis that separates proteins based on their isoelectric pH.
• An electrophoresis that combines both SDS-PAGE and isoelectric focusing is called two-dimesional gel electrophoresis.
• Agarose Gel electrophorises or AGE is used to analyze and determine the molecular weights of nucleic acids, such as DNA and RNA.