microscopy

• Light microscope


  • Coarse supporting the stage
  • Arm connects bottom to stage
  • Light source's mirror reflects the light from
  • Stage where microscope slide is placed
• Objective lenses


  • Different magnifications
  • e.g. 4x, 10x, 50x magnifications
• Eyepiece lens


  Provides further magnification
• Body tube


  • Coarse focusing knob
  • Fine focusing knob
  • Used to get image in focus
• Image


  What we see when we look down the microscope
• Object


  The real object or sample you're looking at
• How microscopes work


  1. Light from room hits mirror and is reflected upwards through the object we want to look at

  2. Passes through one of the objective lens

  3. Passes through eyepiece lens

  4. Into our eye -the lenses spread out light rays so the imagine we see is far larger than the object
• Lenses


  Spread out light rays
• The image we see is 450 times larger than the object
• Magnification


  How many times larger the image is than the object
• Resolution

  The shortest distance between two points on an object that can still be distinguished as two separate entities
• The higher the resolution, the more detailed the image and the less blurry it will be
• Electron microscopes can produce higher magnifications without going blurry
• Electron microscope images are black and white
• • Magnifying power of light microscope is up to x2000
  • Magnifying power of electron microscope is over x2,000,000
• Electrons have a shorter wavelength than light waves
• Electron microscopes can only view clean specimens
• Units of length

  • m
  • mm
  • μm
  • nm