Laboratory

• Antioxidant capacity


  The ability of a substance to neutralize or scavenge free radicals and reactive oxygen species
• Total phenolic content


  The total amount of phenolic compounds present in a sample
• DPPH scavenging assay


  • Evaluates the antioxidant capacity of plant extracts
• Folin-Ciocalteu method


  • Quantifies the total phenolic content in plant extracts
  • Based on the oxidation of phenolic compounds by the Folin-Ciocalteu reagent to form a blue chromophore
• Free radicals can develop naturally within the body through metabolic processes or be introduced from external sources such as cigarette smoke, pollution, and radiation
• DPPH scavenging assay

  1. Add sample to DPPH solution

  2. Measure absorbance at 515 nm

  3. Calculate percent remaining and percent inhibition

  4. Determine IC50 value
• Half-maximum Inhibitory concentration

  The potency of a substance that inhibits a specific biological or biochemical process by 50%
• Absorption spectrophotometer


  An instrument that measures the absorption of electromagnetic radiation by a sample as a function of frequency or wavelength
• Beer-Lambert law


  The absorbance of light by a substance is directly proportional to the concentration of the substance and the path length of the light through the substance
• Plant extracts

  • B. rotunda rhizome extracts (Ethyl acetate, Methanol)
• DPPH scavenging assay reagents

  • 0.1 mM DPPH reagent
• The study aimed to investigate the antioxidant capacity and quantify the total phenolic content of extracts from Fingerroot rhizomes
• DPPH

  A stable free radical used to assess the antioxidant capacity of substances
• DPPH assay


  1. Measures the ability of antioxidants to neutralize DPPH radicals

  2. Indicated by a decrease in absorbance at 515 nm
• Methanol, Ethylene 


  A solvent used to dissolve both the DPPH and the samples being tested for antioxidant activity
• Folin-Ciocalteu reagent


  Is used to quantify phenolic compounds<|>It reacts with phenols to produce a blue-colored complex that can be measured spectrophotometrically at 765 nm
• Sodium carbonate


  An alkaline solution used to create the optimal pH for the reaction between the Folin-Ciocalteu reagent and phenolic compounds
• Gallic acid


  A type of phenolic acid used as a standard to create a calibration curve for the quantification of total phenolic content<|>The results are often expressed as gallic acid equivalents