during prophase , two pairs of centrioles become visible which move to opposite ends of cell and microtubules begin to radiate from them. the nucleolus disappears and nuclear membrane begins to break down. chromatin threads of DNA become tightly coiled and are now seen as chromosomes. by the end of prophase, centrioles have reached opposite poles, some microtubules join to form framework of fibres called spindle, the nuclear membrane has completely disappeared and chromosomes migrate towards equator of the cell