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nucleic acids
semi conservative replication
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Cards (14)
What mechanism is used in DNA replication?
Semi-conservative replication
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What is the role of DNA helicase in DNA replication?
It unwinds and
unzips
the DNA double helix
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What holds the two strands of the DNA double helix together?
Hydrogen bonds
between
bases
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What happens to the hydrogen bonds during DNA replication?
They
break
, allowing the
strands
to
separate
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What do free nucleotides do during DNA replication?
They pair with
exposed bases
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What is complementary base pairing?
Adenine
pairs with
thymine
,
cytosine
with
guanine
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What enzyme catalyzes the formation of phosphodiester bonds?
DNA polymerase
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What is the sugar phosphate backbone in DNA?
It is formed by
phosphodiester bonds
between
nucleotides
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Why is DNA replication called semi-conservative?
Each new DNA molecule has one old and one new
strand
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What are the steps of DNA replication?
DNA unwinds and unzips by
DNA helicase
Hydrogen bonds
between bases break
Free nucleotides pair with
exposed bases
DNA polymerase forms
phosphodiester bonds
New sugar phosphate backbone is created
Resulting DNA molecules have one old and one
new strand
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What nitrogen isotopes were used in the famous experiment for DNA replication?
N-14
and
N-15
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What was observed after the first cycle of replication in the nitrogen experiment?
A mix of
N-14
and
N-15
molecules
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What was the result after the second generation in the nitrogen experiment?
A mix of
N-14
,
N-15
, and some N-15 only
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How does the experiment with nitrogen isotopes provide evidence for semi-conservative replication?
It shows that each DNA molecule contains one
old strand
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