LAB 8

• Procedure A

  1. Make two muscle-nerve preparations and keep them moist with NSS

  2. Place the nerve of specimen A on top of specimen B. Stimulate the nerve of specimen B with single induction shocks of moderate intensity. Note the effects on the muscles of both specimens
• Preparation

  Define the following: Membrane potential, Action potential, Action current, Demarcation current
• Experiment 8

  Action current; The rheoscopic preparation; Current of injury
• Procedure B

  1. Make two muscle-nerve preparations and keep them moist with NSS

  2. Cut a portion of the muscle of preparation B so that it now presents an injured and an uninjured portion

  3. Place the nerve of preparation A on top of preparation B in such a way that the middle of the nerve rests on the injured surface while the end of the nerve rests on the uninjured surface. Note what happens
• Report and Conclusion

  1. Action current: Resting membrane potential, Action potential, Depolarization, Repolarization

  2. Current of injury: Injured membrane, Electronegative damaged area, Demarcation potential, Depolarization but not repolarization