1. HOMOGENISATION

• briefly explain what homogenisation is?

  Breaking up the cells
• give examples of different ways that homogenisation can be done?

  • vibrating the cells
  • grinding the cells up in a blender
• what does homogenisation do?

  Breaks up the plasma membrane and releases the organelles into solution
• why must the solution be kept ice-cold?

  To reduce the activity of enzymes that break down organelles
• What does isotonic mean?

  The solution should have the same concentration of chemicals as the cells being broken down
• why must the solution be isotonic?

  To prevent damage to the organelles through mitosis
• Explain the criteria for the solution?

  • kept ice-cold
  • isotonic
  • buffer added