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RG Analysis of cell components
Cell Fractionation
1. HOMOGENISATION
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Cards (7)
briefly explain what homogenisation is?
Breaking up
the
cells
give examples of different ways that homogenisation can be done?
vibrating
the
cells
grinding
the
cells
up in a
blender
what does homogenisation do?
Breaks
up the
plasma membrane
and
releases
the
organelles
into
solution
why must the solution be kept ice-cold?
To
reduce
the
activity
of
enzymes
that
break down organelles
What does isotonic mean?
The
solution
should have the same
concentration
of
chemicals
as the
cells
being
broken down
why must the solution be isotonic?
To prevent
damage
to the
organelles
through
mitosis
Explain the criteria for the solution?
kept
ice-cold
isotonic
buffer
added
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