Microbiome is the completecollection of microorganisms and their genes within a particular environment
Microbiata is the individualmicrobial species in a biome - bacteria, fungi, archaea and viruses
culture dependent methods relies on culturing of microbes in the lab and uses pure cultures, or simpleenrichments
the pros of culture dependent methods
you can study one organism at a time, allowing access to the phenotype. can manipulate the conditions to see the response of the organism
the cons of dependent methods
there is too many species to grow them all and notall of them can be cultured. culturing requires preciseconditions to match microbes needs and doesn't match the realworld conditions
culture independent methods relies predominantly on nucleic acid based methods. no culturing method required. it uses sequencing or metabolic profiling to study allmicrobes in a sample
pros of culture independent methods
Can study many organisms at a time and shows the communities as they are in nature. It allows access to genotype. Can target non-culturable organisms as well as provides access to unknowninformation or species
cons of cultural independent methods
there is no pure culture, so no ability to manipulate. it is also expensive and complexmethods
