PARA LAB 1-9

• the term used when objectives stays in focus when magnification is changed?

  Parfocal
• What is the formula for total power magnification?


  objective magnification x eyepiece magnification (10x)
• Total magnification of Scanner?

  40x
• Magnification of scanner?


  4x
• Total magnification of HPO?

  400x
• magnification of HPO?


  40x
• Total magnification of LPO?

  100x
• Magnification of LPO?


  10x
• Total magnification of Oil Immersion Objective?

  1000x
• Magnification of OIO?


  100x
• The distance between the front edge of the objective lens and the specimen surface (with the surface of the cover glass in case of the cover glass objective lens) when the specimen is focused.?

  Working distance?
• The compound light microscope

  A) Eyepiece

  B) Arm

  C) Course adjustment knob

  D) Fine adjustment knob

  E) Revolving nose piece

  F) Objective lenses

  G) Stage Cup

  H) Mechanical Stage

  I) Iris diagphragm

  J) Light source

  K) Light adjustment knob

  L) Base

  
• In transporting the microscope what part are you going to hold?

  Arm and base
• Part of the microscope that holds the oculars and can be adjusted for the interpupillary distance ?

  Ocular tube
• Distance between your eyes is called?

  Interpupillary distance
• Other term for revolving nose piece?

  Turret
• the knob that moves the slide horizontally on the mechanical stage found on the upper part?


  x stage control knob
• The knob used to move the slide vertically found on the lower part?


  Y stage control knob
• oil used as an immersion oil in the microscope?

  Cedar oil
• solution for Micrometry calibration in stage micrometer?


  no of stage space x 0.1

  ————————————- x 1000 = value of 1 ocular space in um

  no of ocular space
• solution for calibrating the hemacytometer?


  no of stage space x 0.05

  ——————————————- x 1000 = value of 1 ocular space in um

  no of ocular space
• what is the allowed difference between the values obtained using the stage micrometer and the hemacytometer under the 3 objectives?

  0.1
• Formerly known as Cercomonas lamblia?

  Giardia lamblia
• the parasite that causes beaver fever and traveller's diarrhea?

  G.lamblia
• G.lamblia trophozoite:

  • bilaterally symmetrical, pear shaped, rounded
  • Cytoplasm: grayish voilet, granular
  • Sucking disc: Ventral - concave; Dorsal-convex
  • Nucleus: 2, central dark karyosome
  • Parabasal body - blue black, sausage shaped
  • Axoneme
  • Flagella - 4 pairs
• Infective form of G.lamblia?

  Mature quadrinucleated cyst
• Habitat of G.lamblia?


  Small intestine
• Motility of G.lamblia?


  Falling leaf motion
• Cyst of G.lamblia:

  • Ovoid, football shaped
  • definite cystic wall
  • Nuclei - 1-4 spherical shape (double wall appearance)
• Cercomonas hominis?


  Chilomastix mesnili
• Tropozoite of C.mesnili:

  • Asymmetrically pear-shaped
  • Cytoplasm - grayish voilet, vacuolated
  • Nucleus-single, spherical
  • Spirall groove - obliquely along the ventral surface
  • Flagella - 3
• infective stage of C.mesnili?

  Mature cyst
• Habitat of C.mesnili?


  Colon
• Motility of C.mesnili?


  Stiff and Rotary motion
• Cyst of C.mesnili:

  • Lemon shaped with a nipple like protuberance at the anterior portion
  • Definite cystic wall
  • Nucleus: 1
• I&H stain of C.mesnili cyst:

  • Grayish violet and granular
  • Nucleus and cytostome are clearly seen
• Pentatrichomonas hominis?


  Trichromonas hominis
• Trophozoite of Trichomonas hominis:

  • pyriform, pear like
  • Cytoplasm: GV - finely granular
  • Nucleus: Ovoidal, single, anterior end, central karyosome
  • Cytosomal cleft
  • Full undulating membrane
  • Axostyle protruding
  • FlagellaL 3-4
• Infective stage of T.hominis?

  Mature trophozoite
• Habitat of T.hominis?


  Cecal area of Large intestine