MicroPara

Subdecks (10)

Cards (848)

  • Anton Van Leeuwenhoek
    Dutch microbiologist, Father of Microbiology, Observed pond water and scrapings from his teeth
  • Robert Hooke
    English Scientist, Coined the term "cell", Invented the simplest microscope through illumination principle
  • Types of Microscope
    • Simple Microscope
    • Compound Microscope
    • Electron Microscope
  • Simple Microscope
    • Simplest type of microscope, Uses concave mirror, Does not contain lamp source
  • Compound Microscope
    • Routine microscope used in clinical use, Uses two sets of lenses to obtain higher magnification, Can or cannot contain own lamp source
  • Electron Microscope
    • Most advanced microscope, Used for quality control and failure analysis, Used mainly with microorganisms
  • Classifying Parts of the Microscope
    • Mechanical Parts
    • Illuminating and Magnification Parts
  • Magnification Objectives
    Lenses that further magnifies the microscopic specimens that are not visible to the naked eye
  • Magnification Objectives
    • SCANNER (Adds 4X)
    • LOW POWER OBJECTIVE (LPO) (Adds 10X)
    • HIGH POWER OBJECTIVE (HPO) (Adds 40X)
    • OIL-IMMERSION OBJECTIVE (OIO) (Adds 100X)
  • Ocular Lens/ Eyepiece
    Lens that is closest to the eyes, Can be 10X or 5X magnification
  • Body Tube
    Part of the microscope that houses the oculars/ eyepiece
  • Revolving Nosepiece
    Turret-like circular mechanism that holds the objectives
  • Stage
    Rectangular platform that exhibits the specimen
  • Stage Clips
    Mechanism that keeps the slides in place
  • Condenser
    Concentrates light that can be found between the stage and the lamp
  • Lamp/ Light Source
    Main illuminating mechanism of the microscope, Can be separated or incorporated in the microscope
  • Diaphragm
    • Iris Diaphragm
    • Disk Diaphragm
  • Iris Diaphragm

    Disk Diaphragm that constricts or dilates
  • Disk Diaphragm
    Rotating disk with holes of different diameters
  • Arm
    Bar that supports the upper assembly of the microscope
  • Base
    Square or Horseshoe-shaped support of the microscope
  • Pivot
    Allows the upper assembly to move in a more comfortable viewing position
  • Knobs
    • Course Adjustment Knob
    • Fine Adjustment Knob
  • Course Adjustment Knob
    Adjusts the distance between the objectives and the stage
  • Fine Adjustment Knob
    Used for sharp and fine focusing of the objects
  • Glass Slides
    Rectangular glass materials where the specimens are mounted
  • Coverslips/ Cover glass
    Small square-shaped materials that used to cover the specimens after mounting
  • Lens Cleaning Solution
    Liquid that is used to clean objectives; makes it free from foreign objects that might interfere in the view
  • Lens Cleaning Paper
    Soft material that is used to wipe excess solution from the lens
  • Stains
    Solutions that are used to visualize microscopic specimens
  • Microscopic Etiquette
    • Carry the microscope in an upright position
    • One hand should be in the base, and one hand in the arm
    • Properly uncoil the lamp cord
    • Always focus first using the scanner position/ Low-power objective (LPO)
    • Always put the prepared slides in the center of the stage
    • Use the coarse adjustment knobs while using lower objectives
    • Use the Fine Adjustment knobs while using higher objectives
    • Always keep both of your eyes open while viewing the specimen
  • Specimen Troubleshooting
    • Always keep the diaphragm open
    • Check if there are any obstructions in the objectives, eyepiece, or the stage itself
    • Make sure that the lamp is on
    • Make sure that the specimen is always placed at the center
    • Always clean the lenses before using it. Make sure to use the correct objective cleaning solution to avoid scratches
    • Make sure that the objectives are properly seated
  • Image Fading Troubleshooting

    • Make sure that the scope does not require a rubber eyecup for proper viewing
    • Make sure that the specimen is not in the medium that obstructs viewing
    • Watch to see if the body tube or stage is shifting or drooping
    • Check the lamp or cord for short circuits
  • Wet Mount Slide
    Slide on which a wet specimen is placed, then covered with a coverslip
  • Staining
    Process of enhancing the distinguishing features of the specimen viewed under the microscope
  • Nationally Notifiable Parasitic Diseases   
    Cryptosporidiosis
    Cyclosporiasis
    Giardiasis
    Malaria
    Trichinellosis