Ch6 expression regulation

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    Created by
    BreakableGavial2499

    Cards (25)

    • Expression regulation by RNA degradation involves protein degradation, protein stability, protein synthesis, and mRNA degradation.
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    • Prokaryotes (P) and Eukaryotes (E) differ in mRNA degradation, mRNA abundance, mRNA stability, and mRNA synthesis.
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    • Dhh1p couples translation to mRNA decay by sensing codon optimality.
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    • Non-stop decay (NSD) was first observed on 1819/11/2023.
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    • The DEAD-box protein Dhh1p associates with the translating mRNP.
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    • The codon-dependent rate of translational elongation impacts mRNA stability.
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    • mRNA analysis can be done specifically through Northern blotting, qPCR (Real-time RT-PCR), and high-throughput methods such as transcriptomics.
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    • mRNA turnover can be measured through Pulse-chase, RNA pol II inactivation + cDNA labeling, and RNA pol II inactivation + RNA-seq.
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    • mRNA can be converted to cDNA with specific primer and reverse transcriptase (RT) and PCR-amplified with two specific primers (Taq polymerase or Tth polymerase).
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    • The amplification products in real-time PCR can be monitored using fluorescence detection and the mRNA abundance can be calculated using a standard curve.
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    • Not1 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • PAN3 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • Caf130 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • Not3 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • Not5 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • PABP is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • Caf44 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • PARN is a mRNA degrading enzyme that is inhibited by PABP and stimulated by 5’ cap.
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    • PAN2 is a mRNA degrading enzyme that is stimulated by PABP and has no effect of 5’ cap.
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    • Not4 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • Not2 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • Pop2 is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • m7G is a mRNA degrading enzyme that is inhibited by PABP and has no effect of 5’ cap.
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    • Fam) at the 5' end, Q: Quencher dye (Tamra) at the 3' end, R: Reporter, Q: Quencher, TaqMan® Probe, R: PCR, #: Taq, TaqMan® Probe, R: PCR, #: Taq,
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