Exercise 3

• Bait - a piece of the substrate where the target fungi thrives (e.g. dung)
• Arrange the Sequencing (Isolation and culture of thraustochytrids)

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• Arrange the Sequencing (Isolation and culture of dung fungi)

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• The life cycle of Pilobolus begins with a black sporangium that has been discharged onto a plant substrate such as grass
• The Pilobolus sporangium survives the passage through the gastrointestinal tract without germinating and emerges with the excrement
• The organism in the image belongs to the genus, Pilobolus

  
• Thraustochytrids belong to the Kingdom Chromista
• Thraustochytrids belong to the phylum Stramenopiles
• Thraustochytrids - single-celled saprotrophic eukaryotes that are widely distributed in marine ecosystems, and which secrete enzymes including amylases, proteases, and phosphatases
• Thraustochytrids have lipid rich cells
• Vegetable Juice agar contains: Vegetable juice, Calcium carbonate, Seawater, and Agar
• Glucose-Yeast Extract-Peptone Agar contains: Glucose, Yeast Extract, Peptone, Agar, and Seawater
• Thraustochytrids produce Polyunsaturated fatty acids, which include: Docosahexaenoic Acid, Arachidonic acid, and Eiosapentaenoic acid