Reiner and Gearhart (2004) studied 14 children born with cloacal exstrophy (a severe birth defect where much of the abdominal organs are exposed, often causing the splitting of both male and female genitalia) who were reassigned female at birth (born genetically male)
Follow-up between the ages of 5 to 16 showed that 8 of the 14 who were reassigned identified themselves as boys (i.e. their gender identity matched their genetic sex)
This provides some support for the argument that biological variables have an impact on gender identity and behaviour, independent of socialisation (nurture)
David Reimer's biological sex was male (XY), but despite being raised as a female and receiving female hormone treatment, he never fully came to identify himself as a female, and at the age of 13 reverted to his true sex- male
It has been argued that the size of the SDN (Sexually Dimorphic Nucleus) is determined by levels of testosterone in the womb, and as David was exposed to normal levels of testosterone in the womb, his brain was arguably masculinised